Anti-CFP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P27918

Core Product Specifications and Parameters

Biological Background: Properdin (CFP) Function and Localization

• Properdin, also known as complement factor P, is a positive regulator of the alternative pathway of complement activation. Related references: PMID:16301317, PMID:20382442, PMID:28264884
• It binds to and stabilizes the C3- and C5-convertase enzyme complexes, enhancing complement activation. Related references: PMID:16301317, PMID:20382442, PMID:28264884
• Properdin inhibits the CFI-CFH mediated degradation of complement C3 beta chain (C3b), thereby stabilizing the convertase. Related references: PMID:31507604
• The protein is secreted into the extracellular space, where it exerts its immune functions.
• Properdin is a glycoprotein with multiple disulfide bonds, belonging to the thrombospondin type-1 repeat-containing protein family.
• It has been implicated in disease variants and is a recognized target in proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region of properdin (aa 190-469), which may generate antibodies recognizing both full-length and potential isoforms.
• For Western blot (WB), the predicted protein weight is approximately 51-53 kDa; validate with appropriate positive controls.
• For IHC-P and IF/ICC, consider using signal amplification due to the secreted nature of the target; tissue sections from liver (a major source of complement proteins) may provide positive staining.
• Cross-reactivity is reported for human, mouse, and rat; verify in your specific model.
• ELISA can be used for quantitative detection, but optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For complement system research, we provide high-quality antibodies against properdin and other complement factors, supporting diagnostic development from concept to clinic. Our global supply network enables efficient sourcing of even rare targets.