Anti-CFL2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y281

Core Product Specifications and Parameters

Biological Background: Cofilin-2 Function and Localization

• Cofilin-2 (CFL2), also known as muscle cofilin, is an actin-binding protein that controls reversible actin polymerization and depolymerization in a pH-sensitive manner, binding G- and F-actin in a 1:1 ratio.
• Its F-actin depolymerization activity is regulated by association with CSPR3 (cysteine and glycine-rich protein 3), a muscle-specific LIM domain protein. Related references: PMID:19752190
• CFL2 is a major component of intranuclear and cytoplasmic actin rods and is required for skeletal and cardiac muscle maintenance.
• Two major isoforms exist: CFL2b is predominantly expressed in skeletal muscle and heart, while CFL2a is found in various tissues.
• Subcellularly, CFL2 localizes to the nucleus matrix, cytoplasm, and cytoskeleton, consistent with its roles in actin dynamics and nuclear architecture.
• Key post-translational modifications include acetylation and phosphorylation, and the protein is linked to nemaline myopathy through disease variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human CFL2 (amino acids 1–166), so the antibody is expected to recognize both isoforms.
• For Western blotting, a band near 19 kDa is anticipated; validate using lysates from skeletal muscle or heart tissue where CFL2 is abundant.
• In immunofluorescence, staining patterns should include cytoplasmic filaments and nuclear matrix; co-staining with actin markers is suggested.
• ELISA protocols may be optimized using recombinant CFL2 protein as a standard; cross-reactivity in mouse and rat samples should be confirmed empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, connecting diagnostic manufacturers and research teams with high-quality antibodies like this anti-CFL2 polyclonal for muscle biology and actin cytoskeleton studies. We provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to streamline your workflow from development to validation. For specialized projects targeting rare muscle proteins or neuromuscular disorders, CamelBio offers tailored sourcing and technical support to accelerate your product pipeline.