Anti-CFL1 Rabbit Polyclonal Antibody for WB and ELISA - P23528

Core Product Specifications and Parameters

Biological Background: Cofilin-1 Function and Localization

• Cofilin-1, encoded by the CFL1 gene, is an 18.5 kDa actin-binding protein that regulates actin dynamics by depolymerizing F-actin in a pH-sensitive manner. Related references: PMID:11812157, PMID:33670794
• In early embryonic development, cofilin-1 works with the subcortical maternal complex to facilitate the first cell divisions and ensures symmetric division of zygotes via actin regulation. Related references: PMID:15580268
• It modulates cell morphology and cytoskeletal organization in epithelial cells. Related references: PMID:21834987
• Cofilin-1 promotes the up-regulation of atypical chemokine receptor ACKR2 from endosomes to the cell membrane, enhancing chemokine clearance. Related references: PMID:23633677
• It is required for neural tube morphogenesis and neural crest cell migration (By similarity).
• Subcellular localization includes the nucleus matrix, cytoplasm, and various actin-rich structures such as lamellipodia, ruffles, growth cones, and axons.
• Post-translational modifications include phosphorylation (on Ser3) that inhibits actin binding, as well as acetylation and ubiquitination; it is also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human CFL1; consider that the antibody may recognize an N-terminal epitope, and phosphorylation at Ser3 (within this region) could potentially influence binding.
• For Western blot, a band around 19 kDa is expected; validate the molecular weight against your sample system and consider using appropriate loading controls.
• In ELISA applications, optimize coating conditions and antibody dilutions empirically to achieve optimal signal-to-noise ratio.
• Given cross-reactivity with mouse and rat, the antibody may be suitable for studies in these species; however, sequence alignment of the immunogen region is advisable to confirm epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For the CFL1 target, we offer validated CFL1 antibody pairs, monoclonal antibodies, and ancillary reagents to support your cytoskeleton research and assay development. Our supply capabilities also include bulk raw-material sourcing for rare targets, helping you move from concept to clinic efficiently.