Anti-CFHR5 Rabbit Polyclonal Antibody for WB, ELISA - Q9BXR6

Core Product Specifications and Parameters

Biological Background: CFHR5 Function and Localization

• Complement factor H-related protein 5 (CFHR5) is a secreted protein belonging to the factor H family of complement regulators, encoded by the CFHR5 gene (also known as FHR5, CFHL5).
• CFHR5 functions in complement regulation by competing with the physiological complement inhibitor CFH for tissue-bound complement fragments, thereby modulating the alternative pathway (UniProtKB Q9BXR6).
• The protein can form dimers, which exhibit enhanced avidity for tissue-bound complement fragments, increasing their regulatory efficiency.
• CFHR5 is primarily expressed in the liver and secreted into plasma, consistent with its role in systemic complement control.
• The protein contains multiple sushi (short consensus repeat) domains, which are characteristic of complement regulatory proteins.
• CFHR5 is associated with disease variants, including mutations linked to hemolytic uremic syndrome (HUS), highlighting its clinical significance.
• Post-translational modifications include glycosylation and disulfide bond formation, contributing to its stability and function.

Experimental Guidance and Technical Tips

• Based on the immunogen sequence (amino acids 450-569 of human CFHR5), the antibody is predicted to recognize a specific epitope in the C-terminal region, ideally suited for detecting full-length CFHR5.
• For western blotting, a primary band is expected at approximately 64 kDa; consider using reduced and non-reduced conditions to account for potential disulfide-linked dimers.
• In sandwich ELISA, this polyclonal antibody may serve as either capture or detection antibody; pair with a validated monoclonal antibody for enhanced specificity if needed.
• Validate performance in relevant biological samples such as human serum, plasma, or liver tissue lysates.
• Note that the antibody cross-reacts with mouse and rat CFHR5, but sequence homology should be checked if absolute species specificity is required.
• For best results, optimize dilution factors and blocking conditions empirically in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers, laboratories, and research institutes with high-quality reagents like this CFHR5 antibody. With a focus on complement-related targets, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets—streamlining your development from concept to clinic.