Anti-CFHR3 Polyclonal Antibody for WB, IF-P, ELISA - Q02985

Core Product Specifications and Parameters

Biological Background: CFHR3 Function and Localization

• CFHR3, also designated DOWN16 or H factor-like protein 3, is a member of the complement factor H protein family.
• It is a secreted glycoprotein predominantly expressed by the liver and released into plasma.
• CFHR3 is thought to participate in the regulation of the alternative complement pathway, though its exact role is not fully defined.
• The protein contains multiple sushi (SCR) domains, which are characteristic of complement control proteins and mediate interactions with complement components.
• Disulfide bonds and glycosylation are key post-translational modifications that contribute to protein stability and function.
• Alternative splicing of the CFHR3 gene produces multiple transcript variants, potentially giving rise to isoforms with distinct activities.
• Genetic alterations or deficiency of CFHR3 have been linked to atypical hemolytic uremic syndrome (aHUS), highlighting its involvement in complement-mediated diseases.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 80-200 of human CFHR3. This internal region is expected to generate antibodies against native and denatured protein forms, suitable for multiple detection applications.
• This polyclonal antibody has been reported to work in Western blot (WB), immunofluorescence on paraffin-embedded sections (IF-P), and ELISA. For each assay, optimization using appropriate positive and negative controls is advised.
• Cross-reactivity is documented for human, mouse, and rat, enabling flexibility in selecting disease models. However, sequence homology between species should be verified for the target epitope region.
• For WB, the predicted molecular weight of CFHR3 is approximately 37 kDa; band shifts may occur due to glycosylation. Use appropriate molecular weight markers.
• Consider validating the antibody’s performance in your specific experimental context, as antibody behavior can vary with sample preparation and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of raw materials including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-CFHR3 polyclonal antibody supports complement research and diagnostic assay development, and can be sourced alongside bulk quantities, custom formulations, and rare-target raw materials to streamline your workflow. With end-to-end support from concept to clinic, CamelBio simplifies procurement and accelerates your project timelines.