Anti-CFDP1 Rabbit Polyclonal Antibody for WB and ELISA - Q9UEE9

Core Product Specifications and Parameters

Biological Background: CFDP1 Function and Localization

• Required for the structural stability of pericentromeric heterochromatin; stabilizes CBX5/HP1alpha and H3K9me3 at major satellites and CENPA at minor satellites (PubMed:28367969). Related references: PMID:28367969
• Involved in incorporation of histone H2AZ1/H2AZ into chromatin, contributing to chromatin dynamics (By similarity).
• Promotes binding of guanine-nucleotide releasing factor RCC1 to minor and major satellite repeats, maintaining high GTP-bound RAN levels that facilitate RAN-mediated microtubule nucleation during mitosis (By similarity).
• Plays a role in craniofacial development and is required to maintain normal cell function in embryonic development (By similarity).
• Localizes to the chromosome, centromere, kinetochore, and nucleus, consistent with its heterochromatin-related functions.
• Ubiquitously expressed; alternative splicing produces multiple isoforms.
• Subject to acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond), suggesting regulation by diverse post-translational modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 1–299 of human CFDP1, targeting an N-terminal region; consider this when designing epitope-based assays.
• For western blot, the predicted molecular weight is approximately 34 kDa; validate band specificity using positive and negative controls.
• Polyclonal antibodies may exhibit lot-to-lot variability; standardize protocols and titrate for each new batch.
• Cross-reactivity with mouse and rat allows testing in common rodent models; verify reactivity in your specific tissue or cell line.
• In ELISA, consider using paired detection antibodies or competition assays to confirm target specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, supporting diagnostic developers and research laboratories from early R&D to clinical application. This anti-CFDP1 polyclonal antibody is suitable for investigations into chromatin architecture, centromere biology, and developmental pathways. For bulk procurement or custom antibody development, explore our portfolio of validated monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents tailored to your assay needs.