Anti-CFD Rabbit Polyclonal Antibody for WB, ELISA - P00746

Core Product Specifications and Parameters

Biological Background: Complement Factor D Function and Localization

• Complement factor D (CFD), also known as Adipsin and Properdin factor D, is a serine protease encoded by the CFD gene in humans.
• CFD is the rate-limiting enzyme of the alternative complement pathway, initiating a cascade that leads to pathogen phagocytosis and immune signaling. Related references: PMID:21205667, PMID:22362762, PMID:6769474, PMID:874324
• It cleaves factor B (CFB) when CFB is complexed with C3b, activating the C3 convertase of the alternative pathway, thereby amplifying complement activation. Related references: PMID:21205667, PMID:6769474, PMID:874324
• The protein is secreted and circulates in the blood, functioning in the extracellular environment.
• CFD contains a conserved serine protease domain with a disulfide bond and is synthesized as a zymogen requiring activation.
• Keywords associated with CFD include complement alternate pathway, innate immunity, disease variant, and direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 26–253 of human CFD, covering the mature protein lacking the signal peptide. This design likely yields antibodies that recognize the native secreted form.
• For Western blot, a band around 27 kDa is expected, but consider potential post-translational modifications (e.g., glycosylation) or processing that may alter migration.
• For ELISA, the antibody may be used as a capture or detection reagent. Pairing with another anti-CFD antibody could improve sensitivity; validation in the relevant matrix is advised.
• Because CFD is highly conserved between human and mouse, this antibody may cross-react; verify reactivity if using in mouse models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for diagnostic development. This anti-CFD antibody supports research in complement system disorders and innate immunity. For customized sourcing of rare targets or large-scale manufacturing needs, CamelBio’s one-stop service bridges the gap from concept to clinic.