Anti-CFD Rabbit Monoclonal Antibody for WB, ELISA - P00746

Core Product Specifications and Parameters

Biological Background: Complement Factor D Function and Localization

• Complement factor D (CFD), also known as adipsin, is a serine protease that initiates the alternative pathway of the complement system, enhancing phagocytosis, pathogen breakdown, and adaptive immune signaling. Related references: PMID:21205667, PMID:22362762, PMID:6769474
• It specifically cleaves complement factor B (CFB) when CFB is complexed with C3b, forming the C3 convertase of the alternative pathway and amplifying complement activation. Related references: PMID:21205667, PMID:6769474, PMID:874324
• The alternative pathway is spontaneously initiated by C3 hydrolysis, and CFD acts as the rate-limiting enzyme, linking this cascade to innate immunity.
• Subcellular localization is secreted (extracellular), consistent with its role in serum and tissue microenvironments.
• The protein has a molecular weight of approximately 27 kDa (27033 Da) and contains disulfide bonds that maintain its structure.
• Keywords include serine protease, immunity, innate immunity, and complement alternate pathway.

Experimental Guidance and Technical Tips

• The immunogen spans the active serine protease domain (aa 26–253), so the antibody likely detects both the pro-enzyme and activated CFD in Western blotting under denaturing conditions; a band at ~27 kDa is expected.
• For ELISA applications, pair with a compatible detection antibody; titration may be needed to optimize signal-to-noise ratio.
• This rabbit monoclonal antibody is specific to human CFD; confirm cross-reactivity with other species experimentally if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CFD (adipsin) clone for complement pathway research. Our supply network offers antibody pairs, bulk ancillary reagents, and rare target sourcing, supporting your assay development from early design to clinical application.