Anti-CFAP53 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96M91

Core Product Specifications and Parameters

Biological Background: CFAP53 Function and Localization

• CFAP53, also known as coiled-coil domain-containing protein 11 (CCDC11) or HTX6, is a microtubule inner protein (MIP) integrated into the dynein-decorated doublet microtubules of motile cilia axonemes, essential for ciliary beating (PubMed:36191189)

Related references: PMID:36191189

• CFAP53 regulates the motility patterns of both 9+0 and 9+2 motile cilia by differentially localizing and recruiting axonemal dynein components

• CFAP53 is required for centriolar satellite integrity and the formation of both non-motile primary cilia and motile cilia (PubMed:26538025)

Related references: PMID:26538025

• It is involved in the establishment of organ laterality during embryogenesis through its role in primary cilium beating (PubMed:26531781)

Related references: PMID:26531781

• CFAP53 is essential for sperm flagellum biogenesis and male fertility

• The protein localizes to the cilium axoneme, flagellum axoneme, centrosome, centriole, centriolar satellites, and spindle pole (PubMed:36191189, PubMed:26538025)

Related references: PMID:36191189 PMID:26538025

• Expressed in skin fibroblasts, nasal respiratory epithelial cells, and airway epithelial cells (PubMed:22577226, PubMed:28621423, PubMed:25504577, PubMed:36191189)

Related references: PMID:22577226 PMID:28621423 PMID:25504577 PMID:36191189

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-300 of human CFAP53, covering the N-terminal region; consider that the antibody may detect full-length and truncated isoforms across species.
• For Western blot, a band at approximately 62 kDa is expected; validate in ciliated cell models such as primary human skin fibroblasts (PubMed:22577226) or nasal respiratory epithelial cells (PubMed:25504577) where CFAP53 is endogenously expressed.
• In immunofluorescence/ICC, CFAP53 localizes to ciliary axonemes and centriolar satellites; optimize fixation (e.g., methanol or 4% PFA with Triton X-100) to preserve ciliary structures, and co-stain with ciliary markers like acetylated-tubulin (axoneme) or gamma-tubulin (centrosome) for verification.
• This antibody cross-reacts with mouse and rat CFAP53, making it suitable for rodent models; however, confirm immunoreactivity in the specific tissue or cell line of interest.
• For ELISA applications, as a polyclonal antibody, it can be used as a capture or detection reagent in sandwich assays; perform appropriate titration and validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a leading one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing services. The CFAP53 polyclonal antibody aids research into motile cilia function, flagellar biology, ciliopathies, and developmental processes such as left-right asymmetry. With end-to-end support from concept to clinic, CamelBio empowers diagnostic manufacturers and researchers to accelerate their projects with confidence.