Anti-CFAP44 Polyclonal Antibody for WB, ELISA - Q96MT7

Core Product Specifications and Parameters

Biological Background: CFAP44 Function and Localization

• CFAP44 (Cilia- and flagella-associated protein 44), also known as WD repeat-containing protein 52 (WDR52), is a testis-specific flagellar protein essential for sperm motility.
• It functions in the organization and maintenance of the sperm flagellum axoneme, a microtubule-based structure critical for flagellar bending and movement.
• The protein contains multiple WD repeats and a coiled coil domain, which likely mediate protein-protein interactions within the axonemal complex.
• Subcellularly, CFAP44 localizes to the flagellum axoneme, a component of the sperm cytoskeleton located in the flagellum.
• According to UniProt, the protein is subject to phosphorylation and was identified through proteomics, suggesting dynamic regulation.
• Alternative splicing generates multiple isoforms; the full-length protein has a predicted molecular weight of approximately 214 kDa.

Experimental Guidance and Technical Tips

• For Western blot, given the large predicted molecular weight (~214 kDa), use low-percentage SDS-PAGE gels (e.g., 6–8%) and optimize transfer conditions (e.g., wet transfer with reduced methanol, extended time) to improve detection.
• The immunogen (amino acids 180–370) lies within the N-terminal region; consider this when designing blocking peptides or competition assays.
• In ELISA, the recombinant immunogen fragment can serve as a positive control to confirm antibody specificity; validate titration under your assay conditions.
• Since this is a polyclonal antibody, assess lot-to-lot consistency by including reference standards in each experiment; for quantitative applications, consider affinity purification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive support for IVD raw-material procurement, including validated antibodies like this anti-CFAP44 polyclonal antibody. For sperm biology and fertility research, we offer matched antibody pairs, recombinant proteins, and ancillary reagents to streamline assay development. Contact us to source rare-target antibodies and bulk raw materials tailored to your diagnostic pipeline.