Anti-CFAP20/GTL3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9Y6A4

Core Product Specifications and Parameters

Biological Background: CFAP20/GTL3 Function and Localization

• CFAP20 (Cilia- and flagella-associated protein 20), also known as GTL3, BUG22, EVORF, and fSAP23, is a conserved microtubule inner protein (MIP) essential for motile cilia and flagella function.
• It plays a critical role in axonemal structure organization and motility (PubMed:24414207). Related references: PMID:24414207
• CFAP20 is an integral component of the dynein-decorated doublet microtubules (DMTs) within ciliary axonemes, directly required for motile cilia beating (PubMed:36191189). Related references: PMID:36191189
• The protein regulates cilia size and morphology, influencing ciliogenesis and cellular signaling (PubMed:24414207). Related references: PMID:24414207
• It is necessary for axonemal microtubule polyglutamylation, a key post-translational modification that maintains ciliary stability and mechanical resilience (PubMed:24414207). Related references: PMID:24414207
• Subcellularly, CFAP20 shows a dynamic distribution: it localizes to the nucleus, cytoplasm, centrosome/centriole, cilium basal body, and prominently along the axoneme of cilia and flagella, reflecting its diverse functional roles.
• The UniProt record for CFAP20 (Q9Y6A4) includes keywords such as 3D-structure, cilium, flagellum, microtubule, and nucleus; its high-resolution structures and proteomics-based identification underscore its importance in cell biology and ciliopathy research.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of CFAP20 is 23 kDa; a band around this size should be observed. Consider using appropriate loading controls and validate with tissue/cell lines expressing CFAP20 (e.g., ciliated cell types).
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; check ciliated tissues such as trachea, fallopian tube, or testis sections where CFAP20 is enriched in axonemes.
• The immunogen is a synthetic peptide from the central region (a.a. 94–193); this region may be conserved across human, mouse, and rat, consistent with the reported cross-reactivity. Run sequence alignment to confirm epitope conservation if cross-species reactivity is critical.
• ELISA performance can be optimized using recombinant CFAP20 protein or peptide coating; consider using blocking strategies to reduce background.
• As a rabbit monoclonal antibody, batch-to-batch consistency is high, suitable for long-term studies; however, initial titration is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to high-quality IVD raw materials like this CFAP20/GTL3 rabbit monoclonal antibody. Specializing in antibodies for cytoskeletal and cilia biology research, CamelBio provides validated monoclonal antibodies, bulk production, and complementary ancillary reagents. For custom antibody development or rare target sourcing, CamelBio's comprehensive supply chain and technical services accelerate your project from concept to clinic.