Anti-CFAP20 (C16orf80) Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y6A4

Core Product Specifications and Parameters

Biological Background: CFAP20 (C16orf80) Function and Localization

• CFAP20 is a cilia- and flagella-specific protein also known as basal body up-regulated protein 22 (BUG22) and transcription factor IIB (TFIIB)-related protein.
• It functions as a microtubule inner protein (MIP) within dynein-decorated doublet microtubules (DMTs) of the ciliary axoneme, essential for motile cilia beating. Related references: PMID:36191189
• Required for axonemal microtubule polyglutamylation, a post-translational modification critical for ciliary motility. Related references: PMID:24414207
• Plays a role in regulating the size and morphology of cilia. Related references: PMID:24414207
• Subcellularly localizes to the nucleus, cytoplasm, centrosome, centriole, cilium basal body, and axoneme of motile cilia and flagella.
• Keywords include cilium, flagellum, microtubule, and cell projection, reflecting its specialized role in ciliary biology.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 94–193 of human C16orf80 (CFAP20); consider using a blocking peptide to confirm specificity in Western blot.
• For IF/ICC, permeabilization may be required to detect the axonemal and centrosomal localization; validate with appropriate controls.
• The antibody cross-reacts with mouse and rat; verify sequence homology for your specific model system.
• Since CFAP20 localizes to cilia and flagella, ensure the cell type or tissue expresses motile cilia for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, supporting projects from concept to clinical application. This anti-CFAP20 polyclonal antibody is part of our expanding portfolio for ciliary and flagellar research, complementing our range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For rare-target sourcing and custom reagent procurement, contact our team to explore tailored solutions.