Anti-CETN3 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O15182

Core Product Specifications and Parameters

Biological Background: CETN3 Function and Localization

• CETN3 encodes centrin-3, a member of the centrin family of calcium-binding proteins characterized by EF-hand domains. It is also known as CEN3 or CDC31 homolog.
• Centrin-3 plays a fundamental role in microtubule-organizing center structure and function, particularly at the centrosome and centrioles.
• As a component of the TREX-2 complex, centrin-3 is involved in the export of mRNAs from the nucleus to the cytoplasm through the nuclear pore complex.
• The protein localizes to multiple subcellular sites including the cytoplasm, cytoskeleton, centrosome, centriole, nucleolus, nuclear envelope, and nuclear pore complex.
• Centrin-3 is a phosphoprotein and calcium-binding protein, participating in cell cycle regulation, cell division, and mitosis.
• The protein is implicated in mRNA transport, protein transport, and translocation processes.
• Keywords associated with CETN3 include: Calcium, Cell cycle, Cell division, Cytoplasm, Cytoskeleton, Metal-binding, Mitosis, mRNA transport, Nuclear pore complex, Nucleus, Phosphoprotein, Protein transport, Reference proteome, Repeat, Translocation, Transport.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody is a recombinant fusion protein corresponding to the full-length human CETN3 protein (amino acids 1-167). Consider verifying the antibody's specificity in your experimental system using appropriate controls.
• For western blot (WB) applications, a band at approximately 20 kDa is expected. Optimize loading controls and consider using reducing conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), centrin-3 localizes to centrosomes and nuclear pore complexes. Validate staining patterns with relevant markers in the appropriate cell or tissue type.
• Cross-reactivity with mouse and rat has been reported, making this antibody suitable for studies in common model organisms. However, it is advisable to confirm reactivity in your specific samples.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials. Whether you need validated antibody pairs, optimized monoclonal/polyclonal antibodies, or bulk ancillary reagents, our comprehensive portfolio supports your project from concept to clinic. For targets like CETN3 involved in cell cycle and nuclear transport, our sourcing expertise can also facilitate procurement of rare or hard-to-find reagents to accelerate your research and development.