Anti-CETN2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P41208

Core Product Specifications and Parameters

Biological Background: Centrin-2 Function and Localization

• Centrin-2 (CETN2), also referred to as caltractin isoform 1, is encoded by the CETN2 gene (alternative names: CALT, CEN2).
• Plays a fundamental role in microtubule organizing center structure and function; required for centriole duplication, correct spindle formation, and regulating cytokinesis and genome stability via cooperation with CALM1 and CCP110.
• Acts as a component of the XPC complex in global genome nucleotide excision repair (GG-NER). Together with RAD23B, it stabilizes XPC and stimulates DNA binding of the XPC:RAD23B dimer, initiating recognition of distorted DNA.
• The XPC complex recognizes a wide spectrum of damaged DNA with helix distortions. UV-induced cyclobutane pyrimidine dimers (CPDs) are initially detected by the UV-DDB complex, which then recruits XPC for repair.
• As a component of the TREX-2 complex, involved in mRNA export from the nucleus to the cytoplasm through nuclear pores.
• Localizes to the cytoplasm, cytoskeleton (microtubule organizing center, centrosome, centriole), and nucleus (nuclear envelope, nuclear pore complex, nucleoplasm).
• Exhibits post‑translational modifications including acetylation, phosphorylation, and isopeptide bond formation; calcium‑binding and metal‑binding properties link it to signalling and structural roles.
• Predicted molecular weight is 19.7 kDa, often detected at approximately 20 kDa in experimental systems.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein encompassing full‑length human CETN2 (aa 1–172), likely recognizing multiple epitopes.
• For Western blot, expect a single band near 20 kDa; use positive and negative cell‑line controls to verify specificity.
• In immunofluorescence/ICC, CETN2 should decorate centrosomes and may yield additional cytoplasmic/nuclear staining; co‑stain with a centrosome marker such as γ‑tubulin for validation.
• For ELISA, employ recombinant CETN2 as a standard and titrate the antibody to optimize signal‑to‑noise ratio.
• Cross‑reactivity with mouse and rat is reported, but confirm performance in the species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one‑stop partner for IVD raw material sourcing, connecting diagnostic developers with validated research reagents and bulk ancillary materials. This anti‑CETN2 polyclonal antibody is suited for centrosome biology, DNA repair, and mRNA export studies, and can be integrated into larger assay development workflows. For expanded needs, we also offer custom monoclonal antibody development, optimized antibody pairs, and rare‑target sourcing to accelerate your project from concept to clinic.