Anti-Ceruloplasmin Polyclonal Antibody for WB, IF/ICC, ELISA - P00450

Core Product Specifications and Parameters

Biological Background: Ceruloplasmin Function and Localization

• Ceruloplasmin is a multifunctional blue copper-binding glycoprotein containing 6-7 copper atoms per molecule. Its primary ferroxidase activity oxidizes Fe2+ to Fe3+ without releasing radical oxygen species, a critical step in iron transport across cell membranes. Related references: PMID:16150804
• It oxidizes ferrous ions for incorporation onto apo-transferrins, catalyzes Cu+ oxidation, and promotes the oxidation of biogenic amines such as norepinephrine and serotonin. Related references: PMID:14623105, PMID:4643313, PMID:5912351
• The protein exhibits glutathione peroxidase-like activity, enabling the removal of hydrogen peroxide and lipid hydroperoxide in the presence of thiols. Related references: PMID:10481051
• Ceruloplasmin also functions as an inhibitor of myeloperoxidase (MPO) peroxidase activity and possesses NO-oxidase and NO2 synthase activities that regulate endocrine NO homeostasis. Related references: PMID:16906150
• Known also as ferroxidase or cuproxidase, this secreted glycoprotein is predominantly expressed by the liver and released into plasma. It features a signal peptide and multiple internal repeats.
• The mature protein has a molecular weight of approximately 122 kDa, with key post-translational modifications including N-glycosylation, disulfide bonds, and phosphorylation (as annotated in UniProt).

Experimental Guidance and Technical Tips

• This antibody is suitable for Western blotting; expect a band around 122 kDa in human, mouse, or rat samples. Validate with appropriate loading controls and cell lines known to express ceruloplasmin (e.g., hepatic cells).
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions in your cell system; ceruloplasmin is secreted, so consider using cells treated with secretion inhibitors or co-staining with ER/Golgi markers if retention is observed.
• In ELISA applications, pair with a suitable capture antibody if using as a detection reagent; cross-reactivity with rodent species can be leveraged for model organism studies.
• The immunogen corresponds to amino acids 366–572, a conserved central region; verify expected reactivity in your target species, as cross-reactivity is documented for mouse and rat but may extend to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers from concept to clinic. Our portfolio includes this anti-ceruloplasmin polyclonal antibody—an essential tool for research into iron metabolism, copper homeostasis, and oxidative stress—alongside validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. Let our supply network streamline your procurement of rare-target raw materials and accelerate your assay development projects.