Anti-Ceruloplasmin Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P00450

Core Product Specifications and Parameters

Biological Background: Ceruloplasmin Function and Localization

• Ceruloplasmin, also known as ferroxidase or cuproxidase, is a secreted copper-binding glycoprotein encoded by the CP gene.
• It exhibits ferroxidase activity, oxidizing Fe2+ to Fe3+ without generating radical oxygen species, which is critical for iron transport across membranes. Furthermore, it catalyzes the oxidation of Cu+ and biogenic amines such as norepinephrine and serotonin. Related references: PMID:16150804, PMID:14623105, PMID:4643313, PMID:5912351
• Ceruloplasmin demonstrates glutathione peroxidase-like activity, capable of reducing hydrogen peroxide and lipid hydroperoxides in the presence of thiols. Related references: PMID:10481051
• It plays a role in nitric oxide homeostasis through NO-oxidase and NO2 synthase activities. Related references: PMID:16906150
• The protein provides Cu2+ ions for the degradation of heparan sulfate chains on GPC1 and inhibits MPO peroxidase activity (By similarity).
• Ceruloplasmin is synthesized in the liver and secreted into the plasma, consistent with its function in systemic iron and copper metabolism.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 366–572 of human ceruloplasmin; therefore, this antibody may detect both full-length and potential splice variants or fragments containing this region.
• For Western blotting (WB), the predicted molecular weight of ceruloplasmin is approximately 122 kDa; consider using an appropriate gel percentage and optimized transfer conditions for high-molecular-weight proteins.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), antigen retrieval and appropriate tissue preparation are recommended. Validate staining patterns in known positive and negative tissues.
• In ELISA, the antibody can be used as either capture or detection reagent. Optimal pairing with a complementary anti-CP antibody should be empirically determined.
• Due to sequence homology, cross-reactivity with mouse and rat ceruloplasmin is expected. Further validation in the relevant species model is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For ceruloplasmin detection in iron metabolism studies or related clinical diagnostics, our supply network supports flexible procurement tailored to your development needs.