Anti-CERS5 Polyclonal Antibody for WB and ELISA - Q8N5B7

Core Product Specifications and Parameters

Biological Background: Ceramide Synthase 5 Function and Localization

• CERS5 (Ceramide synthase 5) is a multi-pass membrane protein of the endoplasmic reticulum, belonging to the Lass family of ceramide synthases.
• It catalyzes the N-acylation of sphingoid bases using fatty acyl-CoAs, with high selectivity for palmitoyl-CoA (C16:0-CoA), a key step in de novo ceramide synthesis. Related references: PMID:16951403, PMID:18541923, PMID:22144673
• CERS5 can also use various sphingoid bases as substrates, including sphinganine, sphingosine, phytosphingosine, and sphinga-4E,14Z-dienine, supporting both de novo and salvage pathways of ceramide production. Related references: PMID:31916624
• The enzyme contributes to lipid homeostasis in pulmonary epithelia, playing a role in surfactant production.
• Post-translational modifications include N-linked glycosylation and phosphorylation sites.
• CERS5 is encoded by the CERS5 gene (previously LASS5) and potentially generates multiple isoforms through alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, optimize the primary antibody dilution using a concentration gradient, and validate in a relevant cell line or tissue expressing endogenous CERS5.
• A blocking peptide corresponding to the immunogen sequence can serve as a specificity control in both WB and ELISA.
• In ELISA, use the synthetic peptide antigen as a positive control to confirm antibody reactivity.
• Consider testing cross-reactivity in mouse and rat samples, as indicated, by comparing signal strengths and potentially performing species-specific blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw-material partner, providing diagnostic manufacturers and researchers with reliable access to validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents. For ceramide synthase and sphingolipid metabolism research, this CERS5 antibody is part of a broader portfolio designed to support your assay development from target identification to clinical validation. Contact CamelBio to explore custom sourcing solutions that streamline your supply chain and accelerate your diagnostic projects.