Anti-CERK Rabbit Polyclonal Antibody for WB, ELISA - Q8TCT0

Core Product Specifications and Parameters

Biological Background: Ceramide Kinase Function and Localization

• Ceramide kinase (CERK) specifically phosphorylates ceramide to produce ceramide 1-phosphate (C1P), a bioactive sphingolipid involved in cell proliferation, migration, and inflammation. Related references: PMID:11956206, PMID:16269826, PMID:19168031
• Exhibits substrate specificity for natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), with a preference for the D-erythro isomer, but shows no activity toward sphingosines or other lipids. Related references: PMID:11956206, PMID:16269826, PMID:17392267
• Binds phosphoinositides, which may regulate its subcellular distribution and catalytic activity. Related references: PMID:19168031
• Localizes primarily at the cell membrane and the trans-Golgi network, suggesting roles in membrane trafficking and lipid signaling.
• Highly expressed in heart, brain, skeletal muscle, kidney, and liver; moderate in peripheral blood leukocytes and thymus; low in spleen, small intestine, placenta, and lung.
• Contains ATP-binding and calcium-binding motifs, requires magnesium for kinase activity, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen sequence corresponds to the C-terminal region (aa 400–end) of human CERK; consider that this region may be absent in certain splice variants.
• For Western blot, whole-cell lysates from high-expressing tissues (e.g., heart, brain) or cell lines overexpressing CERK are recommended as positive controls.
• Cross-reactivity with mouse and rat CERK has been validated; if using these species, confirm sequence homology in the C-terminal epitope region.
• For ELISA applications, optimize coating antigen and antibody dilution using the immunogen peptide or recombinant CERK protein as standard.
• No quantitative performance data is provided; empirical validation in the relevant experimental system is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with integrated access to IVD raw materials, from concept to clinic. Our portfolio of ceramide kinase–related reagents includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare-target raw-material sourcing. Whether you need research-grade antibodies or scalable manufacturing materials, CamelBio facilitates seamless procurement tailored to your development goals.