Anti-Cereblon (CRBN) Rabbit Polyclonal Antibody for WB, ELISA - Q96SW2

Core Product Specifications and Parameters

Biological Background: Cereblon Function and Localization

• Protein cereblon (CRBN) serves as the substrate recognition component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase complex, directing targets such as MEIS2, ILF2, and GLUL for proteasomal degradation. Related references: PMID:26990986 PMID:33009960
• Essential for normal limb outgrowth, CRBN regulates the expression of fibroblast growth factor FGF8 and the stability of key developmental proteins. Related references: PMID:20223979 PMID:24328678 PMID:25043012
• In neurons, CRBN maintains presynaptic glutamate release and cognitive functions by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory synapses. Related references: PMID:18414909 PMID:29530986
• Alternative splicing of the CRBN gene produces multiple isoforms, though their functional distinctions remain incompletely defined.
• CRBN localizes to the cytoplasm, nucleus, and membrane, reflecting its diverse roles in protein quality control and signaling.
• It acts as a negative regulator of TLR4 signaling by binding TRAF6 and ECSIT, thereby preventing ECSIT ubiquitination and downstream immune activation. Related references: PMID:31620128
• Widely expressed with highest levels in the brain, CRBN is associated with neurological disorders including intellectual disability.
• Post-translational modifications involve phosphorylation, zinc binding, and ubiquitin conjugation, highlighting its intricate regulatory control.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1–280) of human CRBN; mapping the epitope may aid in correlating functional domains, especially those for DDB1 and substrate interactions.
• For Western blot, a band near 51 kDa is expected; be aware that post-translational modifications or isoform differences could produce additional bands.
• The polyclonal nature suits ELISA applications, but thorough validation against recombinant protein and endogenous controls is advised to confirm specificity.
• Reported cross-reactivity with mouse and rat requires verification in target tissue; include species-specific lysates and appropriate loading controls.

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CamelBio connects diagnostic manufacturers and research labs with a full spectrum of IVD raw materials, including validated antibodies like this anti-CRBN polyclonal. Whether you need optimized antibody pairs, bulk ancillary reagents, or specialized sourcing for ubiquitin-proteasome and neurobiology targets, our streamlined supply chain supports your project from early development to clinical application.