Anti-CEP83 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y592

Core Product Specifications and Parameters

Biological Background: CEP83 Function and Localization

• CEP83 (Centrosomal protein of 83 kDa), also known as CCDC41, NPHP18, or NY-REN-58, is a coiled-coil domain-containing protein that localizes to the distal appendages of centrioles.
• It plays a critical role in the initiation of primary cilium assembly by collaborating with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.
• CEP83 is a component of the centrosome, specifically the distal appendage region of the centriole, and is essential for ciliogenesis.
• Mutations in CEP83 are associated with nephronophthisis-related ciliopathies, highlighting its importance in kidney function.
• The protein undergoes alternative splicing and is subject to phosphorylation, indicating potential regulation of its activity.
• As a reference proteome component, CEP83 has been extensively identified through proteomics, underscoring its constitutive expression in human cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 34-260 of human CEP83 (NP_057206.2); for Western blotting, expect a band at approximately 83 kDa and use appropriate loading controls.
• As a polyclonal antibody, it may recognize multiple epitopes; validate specificity using knockout or knockdown samples when possible.
• For ELISA applications, perform titration experiments to determine the optimal working concentration.
• When using murine models, verify cross-reactivity with mouse CEP83 as sequence variations might affect detection efficiency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, providing a wide range of validated antibody pairs, bulk ancillary reagents, and rare target raw materials. For centrosome biology and ciliopathy research applications, our CEP83 antibody is supported by a full portfolio of related antibodies, antigens, and customized production services to accelerate your assay development from concept to clinic.