Anti-CEP57L1 Polyclonal Antibody for WB, ELISA - Q8IYX8

Core Product Specifications and Parameters

Biological Background: CEP57L1 Function and Localization

• Centrosomal protein 57-like 1 (CEP57L1), also known as Cep57-related protein (Cep57R), is a member of the CEP57 family of centrosomal proteins.
• Encoded by the CEP57L1 gene (aliases: C6orf182, CEP57R) located on chromosome 6, it is expressed in human tissues.
• May play a role in microtubule attachment to centrosomes, contributing to proper mitotic spindle organization and cell division.
• Localizes to the centrosome, the primary microtubule-organizing center, and is found in the cytoplasm associated with the cytoskeleton.
• Contains coiled-coil domains that likely mediate protein-protein interactions critical for centrosome assembly and function.
• Subject to post-translational modifications, including phosphorylation, which may regulate its activity during the cell cycle.
• Alternative splicing generates multiple isoforms, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-167 amino acids of human CEP57L1. For Western blot, consider using whole-cell lysates from human cell lines known to express centrosomal proteins, such as HeLa or U2OS.
• Due to the polyclonal nature of this antibody, optimization of antibody dilution (e.g., 1:500–1:2000) is recommended to achieve optimal signal-to-noise ratio.
• For ELISA, the recombinant protein used as immunogen may serve as a positive control; include appropriate negative controls (e.g., lysates from cells with CEP57L1 knockdown) to confirm specificity.
• Verify cross-reactivity in other species if needed; currently validated for human samples only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, covering every stage from concept to clinic. This anti-CEP57L1 polyclonal antibody is part of our portfolio for centrosome and cell division research, offering validated reagents for immunoassays. Beyond standard catalog antibodies, we supply bulk ancillary reagents, optimized antibody pairs, and support sourcing rare targets to streamline your assay development.