Anti-CEP55 Polyclonal Antibody for WB, IF/ICC, ELISA - Q53EZ4

Core Product Specifications and Parameters

Biological Background: CEP55 Function and Localization

• CEP55 (Centrosomal protein of 55 kDa), also designated URCC6 (Up-regulated in colon cancer 6), is a coiled‑coil protein encoded by the CEP55 gene (C10orf3, URCC6). Alternative splicing yields multiple isoforms.
• Plays an indispensable role in mitotic exit and cytokinesis by recruiting PDCD6IP and TSG101 to the midbody; not required for microtubule nucleation.
  Related references: PMID:16198290 PMID:17853893
• Required for proper brain and kidney development.
  Related references: PMID:28264986
• Subcellularly localizes to the cytoplasm, centrosome, cleavage furrow, and prominently to the midbody and midbody ring during cytokinesis.
• Subject to phosphorylation and classified as a phosphoprotein.
• Widely expressed in proliferative tissues, with highest levels in testis and cancer cell lines; also detected in embryonic brain, fetal brain ganglionic eminence, kidney tubules, adult brain, cerebellum, and intestine (at protein level).

Experimental Guidance and Technical Tips

• The immunogen spans the N‑terminal 1–260 amino acids of human CEP55, covering a region that may be well‑conserved across species; validate cross‑reactivity in your sample system before quantitative assays.
• For western blotting, consider using whole‑cell lysates from rapidly dividing cells (e.g., HeLa, HEK293) or testis tissue to enrich the target; the predicted molecular weight is ~54 kDa, but verify the band pattern using appropriate controls.
• In immunofluorescence (IF/ICC), staining of mitotic cells can highlight midbody localization; optimize fixation and permeabilization conditions to preserve the midbody structure.
• For ELISA, titrate the antibody and confirm specificity against recombinant protein or peptide; note that polyclonal lots may exhibit slight variation in affinity profiles.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target custom sourcing. For CEP55‑related projects in mitosis, cancer, and developmental biology, we can supply this polyclonal antibody alongside complementary reagents to accelerate your assay development and streamline procurement.