Anti-CEP44 Rabbit Polyclonal Antibody for WB, ELISA - Q9C0F1

Core Product Specifications and Parameters

Biological Background: CEP44 Function and Localization

• Centrosomal protein of 44 kDa (CEP44), also known as HBV PreS1-transactivated protein 3 (PS1TP3), is encoded by the CEP44 gene (KIAA1712). This protein exists in multiple isoforms due to alternative splicing.
• Acts as a centriole-enriched microtubule-binding protein essential for centriole biogenesis. Collaborates with CEP295 and POC1B to drive centriole-to-centrosome conversion by ensuring proper centriole wall formation. Related references: PMID:32060285
• Functions as a linker component maintaining centrosome cohesion. Associates with CROCC and regulates its stability and centrosomal localization. Related references: PMID:31974111
• Subcellularly localizes to the centrosome, centriole, spindle pole, and midbody, consistent with its roles in cell division and centrosome dynamics.
• Classified under keywords including 3D-structure, coiled coil, phosphoprotein, and reference proteome, indicating structural features and regulatory post-translational modifications.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein covering amino acids 1–280 of human CEP44 (N-terminal region). For Western blot (WB), a band at ~44 kDa should be observed; validate using positive control lysates from human or mouse cell lines.
• When performing ELISA, consider using the immunogen as a coating antigen or a known positive sample; titration may be required to optimize signal-to-noise ratio.
• Cross-reactivity with human and mouse CEP44 is reported; verify specificity in your experimental system using appropriate controls.
• For centrosome/centriole studies, immunofluorescence may be attempted; however, this application is not validated for this product, so antibody suitability should be empirically tested.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we serve as your comprehensive one-stop sourcing bridge for IVD raw materials, from concept to clinic. For centrosome biology and centriole biogenesis research, this anti-CEP44 polyclonal antibody is available alongside validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. We supply diagnostic manufacturers, labs, and research institutes with the critical tools needed to accelerate assay development and biomarker discovery.