Anti-CENPF Polyclonal Antibody for WB, ELISA - P49454

Core Product Specifications and Parameters

Biological Background: Centromere protein F Function and Localization

• Centromere protein F (CENPF), also known as mitosin, AH antigen, and kinetochore protein CENPF, is a large coiled-coil protein essential for chromosome segregation during mitosis.
• It is required for kinetochore function and the localization of dynein, LIS1, NDE1, and NDEL1 to the kinetochore, facilitating proper chromosome alignment and segregation.
• Beyond mitosis, CENPF regulates recycling of the plasma membrane by linking recycling vesicles to the microtubule network via association with STX4 and SNAP25.
• CENPF acts as a potential inhibitor of pocket protein-mediated processes, regulating the activity of RB proteins during cell division and proliferation, and plays a role in embryonic cardiomyocyte cell cycle and cardiac lineage commitment.
• The protein localizes to multiple subcellular structures including the cytoplasm (perinuclear region), nucleus matrix, centromere/kinetochore, and the spindle apparatus.
• Post-translational modifications include acetylation, phosphorylation, methylation, and prenylation, which may modulate its function in cell cycle regulation and development.
• CENPF is implicated in ciliopathy (primary ciliary dyskinesia) and is involved in DNA synthesis regulation and skeletal myogenesis.
• It also contributes to dendritic cell-mediated T-cell immunity against chlamydia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region near the C-terminus of human CENPF, which may be suitable for detecting full-length protein and potential isoforms.
• Given the large predicted molecular weight (~358 kDa), use appropriate gel electrophoresis conditions (e.g., low percentage acrylamide gels) and extended transfer times for Western blotting.
• For ELISA, consider using the recombinant protein or cell lysates from human or mouse samples to validate antibody specificity.
• Since CENPF is expressed in dividing cells, ensure to use proliferating cell lines or tissues to observe expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and bulk ancillary reagents. This CENPF polyclonal antibody supports research in cell cycle, chromosome biology, and cancer diagnostics. For rare targets like CENPF, CamelBio offers customized sourcing and supply solutions to accelerate your assay development.