Anti-CENPC Polyclonal Antibody for WB and ELISA - Q03188

Core Product Specifications and Parameters

Biological Background: Centromere Protein C Function and Localization

• Centromere protein C (CENPC) is a core component of the CENPA-NAC (nucleosome-associated) complex, essential for kinetochore assembly and mitotic chromosome segregation.
• Functions in recruiting the CENPA-CAD complex and facilitating incorporation of newly synthesized CENPA histones into centromeric chromatin.
• Recruits DNA methyltransferase DNMT3B to centromeric and pericentromeric satellite repeats, regulating DNA methylation and histone modifications in these regions.
• Localizes to the nucleus, specifically at the centromere and kinetochore structures of chromosomes.
• Belongs to a family of centromere proteins; alternative splicing produces multiple isoforms, contributing to functional diversity.
• Subject to post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, indicative of tight cell-cycle regulation.
• Plays a role in both interphase and mitosis, ensuring accurate chromosome segregation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1-300) of human CENPC, which may target conserved epitopes and yield cross-reactivity with mouse and rat orthologs.
• For Western blot, a band around 107 kDa is expected in lysates from human, mouse, and rat cells. Consider using whole cell or nuclear extracts containing centromeric proteins.
• In ELISA, validate using recombinant CENPC or centromere-enriched fractions. Cross-reactivity with mouse and rat suggests applicability in comparative studies.
• Since CENPC is a nuclear protein, ensure proper nuclear extraction protocols to enrich the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop supply chain for IVD raw materials, connecting researchers and manufacturers with high-quality antibodies like this anti-CENPC polyclonal antibody for centromere and kinetochore research. Beyond individual antibodies, we source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to streamline your assay development from concept to clinical application.