Anti-CENPB Rabbit Polyclonal Antibody for WB, ELISA - P07199

Core Product Specifications and Parameters

Biological Background: CENPB Function and Localization

• CENPB, also known as centromere protein B, is a major centromere autoantigen encoded by the CENPB gene.
• It specifically recognizes and binds the 17 bp CENP-B box motif within alphoid satellite DNA. Related references: PMID:11726497
• Through DNA binding, CENPB organizes centromeric satellite arrays into higher-order chromatin structures.
• This structural organization is essential for directing centromere formation and kinetochore assembly during cell division.
• The protein localizes to the nucleus and is enriched at the centromere of chromosomes.
• CENPB is subject to multiple post-translational modifications including phosphorylation, ADP-ribosylation, methylation, and ubiquitination.
• Its molecular weight is predicted at ~65 kDa, consistent with the detected band size.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 500–C-terminus) of human CENPB; this design may help in detecting full-length protein.
• For Western blotting, a band around 65 kDa is expected; consider using nuclear extracts from human, mouse, or rat cell lines.
• In ELISA applications, the antibody may be suitable for capturing or detecting CENPB; titrate optimally for your assay.
• Cross-reactivity with mouse and rat is reported; validate specificity in your target species and tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop platform for IVD raw-material sourcing, technical services, and consulting—supporting projects from concept to clinical application. This anti-CENPB polyclonal antibody is optimized for chromatin and centromere studies, and we can supply additional validated antibody pairs, bulk ancillary reagents, and customized monoclonal or polyclonal antibodies to accelerate your assay development.