Anti-CENPA Rabbit Polyclonal Antibody for WB, ELISA - P49450

Core Product Specifications and Parameters

Biological Background: CENPA Function and Localization

• CENPA is a histone H3-like variant that replaces conventional H3 in centromeric nucleosomes. Related references: PMID:11756469, PMID:14667408, PMID:15282608, PMID:18072184
• CENPA-containing nucleosomes exhibit altered DNA wrapping, resulting in less rigid and protruding DNA ends compared to canonical H3 nucleosomes. Related references: PMID:26878239, PMID:27499292
• It acts as an epigenetic mark that propagates centromere identity through replication and cell division. Related references: PMID:15282608, PMID:15475964, PMID:20739937
• CENPA is required for the recruitment and assembly of kinetochore proteins, thereby ensuring proper chromosome segregation and mitotic progression. Related references: PMID:11756469, PMID:14667408, PMID:18072184
• Subcellularly, CENPA localizes specifically to centromeres within the nucleus.
• Post-translational modifications include phosphorylation, methylation, ADP-ribosylation, and ubiquitination, which may regulate its function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human CENPA. Verify antibody specificity in the relevant sample system, as cross-reactivity with other histone H3 variants may occur.
• For Western blotting, endogenous CENPA migrates at approximately 16 kDa. Use whole-cell lysates from actively dividing human cells (e.g., HeLa, HEK293) as positive controls, and include appropriate loading controls.
• For ELISA, consider direct or indirect coating with the immunogen peptide. Titrate the antibody over a broad range (e.g., 0.1–10 µg/mL) to determine optimal working concentration.
• Validate detection in your specific experimental context; ensure appropriate blocking and washing steps to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials and technical services to support diagnostic manufacturers, laboratories, and research institutes. For centromere and mitosis research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. Our sourcing capabilities extend to rare targets and bulk raw materials, streamlining your path from concept to clinic.