Anti-Cenexin1/ODF2 Rabbit Monoclonal Antibody for WB, ELISA - Q5BJF6

Core Product Specifications and Parameters

Biological Background: Cenexin1/ODF2 Function and Localization

• Cenexin1/ODF2, also known as outer dense fiber protein 2 or cenexin, is encoded by the ODF2 gene and exists as multiple isoforms via alternative splicing.
• It is a major component of sperm tail outer dense fibers (ODFs), filamentous structures that contribute to the passive elastic properties and motility of sperm.
• As a centrosomal scaffold, Cenexin1/ODF2 localizes to the distal/subdistal appendages of mother centrioles and is required for the recruitment of PLK1 and ninein (NIN) to the centrosome.
• The protein is essential for the assembly and/or maintenance of normal centrin-1 (CETN1) architecture at the centrosome.
• Subcellularly, Cenexin1/ODF2 is found in the cytoplasm, associated with the cytoskeleton, microtubule organizing center, centrosome, centrioles, spindle poles, and within cilia and flagella.
• Testis-specific expression is observed at the protein level, with high abundance in the cytoplasm of round spermatids and localization to the midpiece and principal piece of the sperm flagellum.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, consistent with its regulatory roles in spermatogenesis and cell cycle.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide from the C-terminal region (aa 730-829), which may recognize multiple ODF2 isoforms; use this information when interpreting band patterns in Western blot.
• For Western blot, a specific signal around 95 kDa is anticipated in human testis or sperm lysates, but examine for possible additional bands due to alternative splicing or modifications.
• In ELISA applications, coating with the immunogen peptide or recombinant ODF2 protein may be used; titrate the antibody and appropriate secondary detection reagents to optimize signal-to-noise.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a single-source bridge for diagnostic manufacturers, laboratories, and research institutes requiring premium IVD raw materials and support. For cenexin/ODF2-targeted projects—including fertility diagnostics or centrosome biology research—we offer a comprehensive portfolio ranging from validated monoclonal antibodies like this anti-Cenexin1/ODF2 clone to optimized antibody pairs, bulk ancillary reagents, and specialized sourcing of rare targets. Partner with us to streamline your entire development pathway from concept to clinic.