Anti-CELF5 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N6W0

Core Product Specifications and Parameters

Biological Background: CELF5 Function and Localization

• CELF5 (CUGBP Elav-like family member 5), also known as Bruno-like protein 5 (BRUNOL5) or CUG-BP- and ETR-3-like factor 5, is an RNA-binding protein encoded by the CELF5 gene (Gene ID: 60680) in humans.
• CELF5 is a member of the CELF family of RNA-binding proteins, which regulate various aspects of mRNA processing, including alternative splicing, editing, and stability.
• The primary function of CELF5 is to regulate pre-mRNA alternative splicing by mediating exon inclusion or exclusion in a tissue-specific and developmentally regulated manner. Specifically, it activates exon 5 inclusion of cardiac troponin T (TNNT2) during the juvenile-to-adult heart transition by binding to muscle-specific splicing enhancer (MSE) intronic sites.
• Subcellular localization studies show that CELF5 is present in both the nucleus and cytoplasm, consistent with its roles in mRNA processing and transport.
• Expression of CELF5 is predominantly observed in the brain, suggesting a role in neuronal mRNA metabolism.
• The protein contains multiple RNA recognition motif (RRM) repeats that facilitate its binding to target pre-mRNAs.
• Alternative splicing of CELF5 itself generates different isoforms, which may have distinct functions or expression patterns.

Experimental Guidance and Technical Tips

• The immunogen is designed against the N-terminal region (amino acids 1-45) of human CELF5, which may confer specificity for this particular isoform; consider confirming specificity if multiple isoforms are present in your sample.
• For western blot (WB), the predicted molecular weight of CELF5 is approximately 52 kDa; validate the observed band size in your experimental system.
• In immunofluorescence (IF/ICC) experiments, CELF5 is reported to localize to both nucleus and cytoplasm; appropriate fixation and permeabilization methods should be optimized to preserve antigenicity.
• The antibody has been tested on human and mouse samples; if using other species, cross-reactivity should be verified empirically.
• For ELISA applications, consider pairing with a matched detection antibody to establish a sensitive sandwich ELISA; the polyclonal nature may provide broad reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents, serving diagnostic manufacturers, labs, and research institutes. The CELF5 polyclonal antibody supports investigations into alternative splicing regulation, particularly in cardiac and neuronal contexts, and is available for bulk orders. Contact us for assistance in sourcing rare targets or customized raw-material solutions to accelerate your assay development from concept to clinic.