Anti-CELF2 Polyclonal Antibody for WB and ELISA - O95319

Core Product Specifications and Parameters

Biological Background: CELF2 Function and Localization

• CELF2 (CUGBP Elav-like family member 2), also known as BRUNOL3, ETR-3, NAPOR, and CUGBP2, is an RNA-binding protein that mediates post-transcriptional regulation.
• It regulates pre-mRNA alternative splicing, mRNA translation, and stability. For example, it activates exon 5 inclusion of TNNT2 in embryonic skeletal muscle and controls actinin exon switching. Related references: PMID:11158314, PMID:11931771, PMID:12649496
• CELF2 participates in apoB mRNA editing by binding to AU-rich sequences upstream of the edited cytidine. Related references: PMID:11577082
• It preferentially binds UG-rich motifs, UGUU sequences, and (CUG)n repeats, targeting a wide range of transcripts. Related references: PMID:11158314, PMID:11931771, PMID:15657417
• Together with CELF1, CELF2 negatively regulates miRNA biogenesis, such as pri-MIR140 processing. Related references: PMID:28431233
• The protein localizes to both the nucleus and cytoplasm.
• Tissue expression is broad, with isoform-specific patterns: isoform 1 in brain and lung, isoform 2 in heart, brain, kidney, and skeletal muscle, among others.
• Keywords from UniProt associate CELF2 with disease variants causing epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 422–521 within the C-terminal region of human CELF2. Confirm that this epitope is present in the isoforms relevant to your study.
• For western blot, the predicted molecular weight is 54 kDa. Alternative splicing may generate isoforms of different sizes; include appropriate positive and negative controls.
• This antibody is suitable for ELISA. For quantitative applications, consider performing a pairing study with matched detection antibodies.
• Cross-reactivity is reported for human; reactivity with other species has not been characterized and would need independent validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For RNA-binding protein targets like CELF2, we supply validated polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents. Our network excels in sourcing rare-target raw materials, ensuring your development workflow stays on track with reliable, high-quality inputs.