Anti-CELF1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q92879

Core Product Specifications and Parameters

Biological Background: CELF1 Function and Localization

• CELF1 (CUGBP Elav-like family member 1), also known as CUGBP1, BRUNOL2, or EDEN-BP, is a 52 kDa RNA-binding protein that belongs to the CELF family, characterized by three RNA recognition motifs (RRMs) involved in post-transcriptional gene regulation.
• Functions broadly in pre-mRNA alternative splicing, acting as both activator and repressor; for example, it promotes exon 5 inclusion of TNNT2 (cardiac troponin T) pre-mRNA during heart remodeling and regulates mutually exclusive exons in α-actinin transcripts.
• Regulates mRNA translation and stability, including increasing translation of CEBPB in aging liver and CDKN1A by antagonizing CALR3 repressive effects, and mediates rapid cytoplasmic deadenylation of transcripts containing EDEN elements by recruiting PARN.
• Binds to diverse RNA motifs: (CUG)n triplet repeats in DMPK, Bruno response elements (BREs), AU-rich elements (AREs) in JUN and FOS, and muscle-specific splicing enhancer (MSE) sites in TNNT2.
• Localizes to both the nucleus and cytoplasm, enabling its involvement in nuclear pre-mRNA processing and cytoplasmic mRNA fate determination.
• Plays a role in miRNA biogenesis by binding to primary microRNA pri-MIR140 and, together with CELF2, negatively regulating its processing into mature miRNA. Related references: PMID:28431233
• Post-translationally modified by acetylation and phosphorylation, and subject to ubiquitination (isopeptide bond), which may modulate its activity and stability.
• Widely expressed across human tissues (ubiquitous), reflecting its fundamental roles in RNA metabolism.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning amino acids 108–483, covering a large portion of CELF1 including multiple RNA-binding domains; this polyclonal antibody is likely to recognize multiple epitopes, potentially providing robust detection.
• For Western blot (WB) analysis, a band at approximately 52 kDa (calculated molecular weight) is expected; validate in the relevant sample system using appropriate controls (e.g., knockdown/knockout lysates or peptide blocking).
• For immunohistochemistry (IHC-P), consider antigen retrieval methods (e.g., heat-mediated citrate buffer) and test antibody dilution series, as signal may vary depending on fixation and tissue type.
• In immunoprecipitation (IP) and ELISA, the antibody’s polyclonal nature may offer high avidity, but users should confirm performance under their specific conditions, including cross-reactivity with mouse and rat orthologs.
• Given CELF1’s nuclear and cytoplasmic localization, subcellular fractionation or dual-fluorescence microscopy co-staining could help validate detection specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality IVD raw materials, technical services, and consulting. For studies on RNA-binding proteins and post-transcriptional regulation, CamelBio offers a range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether advancing basic research or developing clinical assays, CamelBio bridges the gap from concept to clinic with reliable supply chains and expert support.