Anti-CEL/Bile Salt-Activated Lipase Polyclonal Antibody for WB, ELISA - P19835

Core Product Specifications and Parameters

Biological Background: CEL Function and Localization

• Bile salt-activated lipase (CEL), also designated BAL, BSSL, carboxyl ester lipase, cholesterol esterase, and sterol esterase, is a secreted serine esterase encoded by the CEL gene (Gene ID: 1056) on chromosome 9.
• CEL catalyzes the hydrolysis of a broad spectrum of substrates, including cholesteryl esters, phospholipids, lysophospholipids, di- and triacylglycerols, and fatty acid esters of hydroxy fatty acids (FAHFAs), with a preference for FAHFAs bearing the ester bond distal to the carboxylate and unsaturated FAHFA species. This activity is essential for complete digestion of dietary lipids and absorption of fat-soluble vitamins in the intestine. Related references: PMID:10220579, PMID:27509211, PMID:27650499
• The protein is primarily produced in the pancreas and lactating mammary gland, secreted into pancreatic juice and milk, and has also been detected in eosinophils and duodenal fluid.
• CEL is a glycoprotein that contains multiple disulfide bonds and an N-terminal signal peptide for secretion. Alternative splicing generates several isoforms, and mutations in the CEL gene are associated with maturity-onset diabetes of the young type 8 (MODY8).
• Keywords from UniProt include secreted, glycoprotein, serine esterase, lipid degradation, disulfide bond, and alternative splicing, reflecting its functional and structural properties.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant immunogen spanning amino acids 350–550 of human CEL (NP_001798.2); it may recognize isoforms and orthologs containing this region, including mouse CEL (reported cross-reactivity).
• For Western blot, consider using pancreatic or mammary tissue lysates, or CEL-transfected cell lines, as positive controls. The predicted molecular weight is 79 kDa, but glycosylation may cause migration shifts; validate in the relevant sample system.
• In ELISA, confirm reactivity against the full-length protein or recombinant fragment and titrate the antibody to determine optimal coating and detection conditions.
• When working with mouse samples, verify binding and optimal dilution, as cross-reactivity has been reported but may differ from human reactivity.
• Optimize assay conditions and include appropriate negative controls to account for polyclonal background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one‑stop sourcing partner for IVD raw materials, offering this anti‑CEL polyclonal antibody alongside a full lipid metabolism and digestive enzyme panel. Our supply capabilities encompass custom antibody generation, bulk‑grade polyclonal and monoclonal antibodies, validated antibody pairs, and ancillary reagents, all backed by technical consultation to accelerate your diagnostic development from concept to clinic.