Anti-CELA2A Polyclonal Antibody for WB, IF-P, ELISA - P08217

Core Product Specifications and Parameters

Biological Background: CELA2A Function and Localization

• CELA2A (Chymotrypsin-like elastase family member 2A), also known as Elastase-2A, belongs to the serine protease family and functions as an elastase.
• It acts as a secreted enzyme that enhances insulin signaling and plays a physiologic role in cellular glucose metabolism, circulating in plasma to reduce platelet hyperactivation and modulate insulin secretion, degradation, and sensitivity.
• The protein is primarily expressed in the pancreas, detected in exocrine secretions and a small fraction of cells in pancreatic islets, adrenal cortex, intestinal glands, and colonic lymphoid follicles, and is also present in plasma.
• Subcellularly, CELA2A is secreted, consistent with its extracellular and systemic regulatory functions.
• Keywords associated with CELA2A include diabetes mellitus, obesity, and direct protein sequencing, highlighting its link to metabolic disease and clinical relevance.
• It is synthesized as a zymogen with a signal peptide and contains disulfide bonds characteristic of serine proteases, requiring activation for full enzymatic activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 80–150 of human CELA2A, a region that may represent a conserved surface epitope; cross-reactivity with mouse and rat should be validated if precise epitope mapping is required.
• For Western blot (WB), a band at approximately 29 kDa is expected; consider running appropriate positive controls (e.g., pancreatic tissue lysates) and use a reducing SDS-PAGE loading buffer.
• In immunofluorescence (IF-P), confirm antibody specificity by comparing staining patterns in CELA2A-positive tissues (pancreas) versus known negative controls, and optimize permeabilization conditions for the secreted protein.
• For ELISA, employ recombinant CELA2A or peptide antigens as coating agents, and validate the assay’s dynamic range and matrix effects when using plasma or serum samples.
• Because CELA2A is secreted, cell culture supernatants or biological fluids are suitable sample types; include a blocking step to minimize non-specific binding in immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CELA2A polyclonal antibody supports studies in diabetes and insulin signaling, and we complement it with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.