Anti-CECR5 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9BXW7

Core Product Specifications and Parameters

Biological Background: Haloacid Dehalogenase-like Hydrolase Domain-containing 5 Function and Localization

• Haloacid dehalogenase-like hydrolase domain-containing 5 (HDHD5), also known as cat eye syndrome critical region protein 5 (CECR5), is a 46321 Da protein encoded by the CECR5/HDHD5 gene in the cat eye syndrome critical region on chromosome 22.
• The protein belongs to the haloacid dehalogenase (HAD) superfamily, characterized by a conserved hydrolase domain; however, its specific substrates and catalytic activity remain uncharacterized.
• Multiple protein isoforms are produced through alternative splicing, as indicated by UniProt keyword annotation.
• A predicted N-terminal signal peptide suggests potential secretion or membrane localization, consistent with the UniProt keyword "Signal."
• HDHD5/CECR5 is widely expressed across human tissues, based on tissue specificity data.
• It has been identified in high-throughput proteomics studies and is part of the UniProt reference proteome set.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 324-423), suitable for detecting both full-length and truncated isoforms in denaturing and native conditions.
• For Western blot (WB), a specific band near 46 kDa is expected; run appropriate positive controls and validate antibody specificity in your system.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize cell fixation and permeabilization protocols, as the target may localize to cytoplasmic or membrane compartments.
• For ELISA, indirect detection formats are recommended; pair this antibody with a suitable secondary conjugate after verifying reactivity in your assay.
• Cross-reactivity with other species is unknown; restrict initial studies to human samples and validate if extension to other species is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, research laboratories, and academic institutes with a comprehensive portfolio of IVD raw materials. For cat eye syndrome and hydrolase research, we offer this validated anti-CECR5 monoclonal antibody alongside customized sourcing services for companion antibodies, recombinant proteins, and ancillary reagents. Whether you need bulk orders, rare target development, or technical consulting from concept to clinic, CamelBio serves as a reliable one-stop procurement partner.