Anti-CEBPG Polyclonal Antibody for WB - P53567

Core Product Specifications and Parameters

Biological Background: CEBPG Function and Localization

• CEBPG is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors.
• It functions as a transcriptional activator by binding to promoter and enhancer regions of target genes.
• It specifically interacts with the enhancer element PRE-I of the IL-4 gene, regulating its expression. Related references: PMID:7665092
• It also binds to the regulatory regions of the immunoglobulin heavy chain, implicating it in B-cell function.
• Additionally, CEBPG recognizes the GPE1 cis-acting element in the G-CSF gene promoter, influencing granulocyte colony-stimulating factor expression.
• Subcellular localization is predominantly nuclear, consistent with its role in transcriptional regulation.
• Annotated keywords include Activator, DNA-binding, Isopeptide bond, Nucleus, Reference proteome, and Ubl conjugation, highlighting its regulatory and post-translational modification features.

Experimental Guidance and Technical Tips

• The antibody was raised against the N-terminal region (amino acids 1–150) of human CEBPG; check for potential cross-reactivity with other C/EBP family members containing similar domains.
• For western blotting, a band around 16 kDa corresponds to the predicted molecular weight; run appropriate positive controls.
• For IHC-P and IF/ICC, test both human and mouse tissues, as cross-reactivity is reported; optimize antigen retrieval and blocking conditions.
• In ELISA applications, titrate the antibody and use an appropriate secondary detection system.
• As a polyclonal antibody, lot-to-lot variability may occur; always include internal controls and validate new lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For transcription factor research like CEBPG, we offer a range of optimized antibodies and can assist with rare target raw-material sourcing to accelerate your assay development. Our one-stop service covers the full development cycle from concept to clinic, ensuring seamless procurement.