Anti-CEBPE Polyclonal Antibody for WB, ELISA - Q15744

Core Product Specifications and Parameters

Biological Background: CEBPE Function and Localization

• CCAAT/enhancer-binding protein epsilon (CEBPE) is a member of the C/EBP family of transcription factors, named also as CRP1, C/EBP-epsilon, and linked to immunodeficiency 108 (IMD108).
• Acts as a transcriptional activator, regulating gene expression during myeloid differentiation. Related references: PMID:26019275
• Recognizes and binds to two distinct DNA motifs: the CCAAT homology sequence found in many promoters, and the enhanced core homology sequence common to many enhancers.
• Essential for the promyelocyte-to-myelocyte transition in myeloid cell development. Related references: PMID:10359588
• Localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Strongest expression is observed in promyelocyte and late-myeloblast-like cell lines.
• Classified as a phosphoprotein and subject to post-translational modifications including isopeptide bond formation and ubiquitin-like conjugation (ISGylation).
• Disease-causing variants are linked to immunodeficiency (IMD108), underscoring its critical immune function.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–190); thus this polyclonal antibody may detect multiple epitopes on CEBPE. Consider using full-length controls to confirm specificity.
• For Western blot, a band at ~31 kDa is expected; include promyelocyte or myeloblast cell lysates (e.g., HL-60, NB4) as positive samples, as these express CEBPE most abundantly.
• The antibody is suitable for ELISA; validate by coating with recombinant CEBPE protein and optimize coating concentrations empirically.
• Cross-reactivity with mouse is noted; for murine samples, confirm reactivity in the target tissue or cell line and adjust blocking conditions as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For CEBPE research—focusing on transcriptional regulation in myeloid differentiation—CamelBio can source rare-target reagents and scale supply to accelerate your assay development from bench to clinic.