Anti-C/EBPB Polyclonal Antibody for WB, IHC-P, ELISA - P17676

Core Product Specifications and Parameters

Biological Background: C/EBPB Function and Localization

• C/EBPB, also designated NF-IL6, TCF5, LAP, and LIP, is a CCAAT/enhancer-binding protein beta transcription factor encoded by the CEBPB gene.
• It critically regulates the expression of genes involved in immune and inflammatory responses, including binding to regulatory regions of acute-phase and cytokine genes (PubMed:12048245, PubMed:1741402, PubMed:18647749). Related references: PMID:12048245 PMID:1741402 PMID:18647749
• Essential for adipogenesis: after phosphorylation-mediated activation, it induces CEBPA and PPARG, thereby driving the transcriptional program for adipocyte differentiation (PubMed:20829347). Related references: PMID:20829347
• Plays additional roles in gluconeogenesis, liver regeneration, hematopoiesis, female reproduction, osteoblast differentiation, and intracellular bacteria killing, with functional diversity arising from interplay with other proteins and regulatory modifications.
• Localizes to both the nucleus and cytoplasm; its activity is governed by post‑translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination, as well as by isoform‑specific interactions.
• The LIP isoform acts as a dominant negative regulator through heterodimerization with the LAP isoform, influencing processes such as osteoclastogenesis (PubMed:11741938). Related references: PMID:11741938
• Expressed at low levels in lung, kidney, and spleen, and is indispensable for macrophage activation and CD4+ T‑cell responses.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight is approximately 36 kDa; however, be aware that multiple isoforms and extensive post‑translational modifications may cause additional bands or shifts.
• For immunohistochemistry (IHC‑P), consider using liver, adipose tissue, or inflamed tissue as positive controls, and validate the signal with a known negative control tissue.
• For ELISA, due to the polyclonal nature of this antibody, include appropriate controls to confirm specificity and consider potential inter‑batch variation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers and research institutes access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. In the area of transcription, inflammation, and adipogenesis research, we connect you with rare‑target raw materials and supply‑chain solutions to support your entire development pipeline.