Anti-C/EBPB Monoclonal Antibody for WB - P17676

Core Product Specifications and Parameters

Biological Background: C/EBPB Function and Localization

• C/EBPB is a member of the C/EBP family of transcription factors, also known as TCF5, IL6DBP, NF-IL6, or C/EBP-beta. Alternative translation initiation produces multiple isoforms, including the full-length liver activator protein (LAP) and the truncated liver-enriched inhibitory protein (LIP), which can heterodimerize and act as a dominant-negative regulator (PubMed:11741938). Related references: PMID:11741938
• C/EBPB is a key transcription factor regulating immune and inflammatory gene expression, essential for CD4+ T-cell response, granuloma formation, and endotoxin shock (PubMed:12048245, PubMed:1741402, PubMed:18647749). Related references: PMID:12048245 PMID:1741402 PMID:18647749
• During adipogenesis, C/EBPB is rapidly induced and, after phosphorylation, activates CEBPA and PPARG to drive the adipocyte phenotype; it also promotes osteoblast differentiation and restricts odontoblast differentiation (PubMed:20829347). Related references: PMID:20829347
• Localizes predominantly to the nucleus but can also be found in the cytoplasm, consistent with its role as a transcription factor.
• Post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination regulate its activity, DNA binding, and protein interactions.
• Tissue expression is low in lung, kidney, and spleen, but its expression is regulated in immune cells and liver.
• Plays essential roles in female reproduction (ovarian follicle development) and early embryogenesis, redundantly with CEBPA.

Experimental Guidance and Technical Tips

• Immunogen design: The immunogen is a synthetic peptide covering amino acids 246–345 of human C/EBPB, a region that may be conserved across isoforms. When designing experiments, consider potential cross-reactivity with different isoforms and validate with isoform-specific controls.
• Application notes for WB: Expect a primary band at ~36 kDa for the full-length LAP isoform; the LIP isoform may appear as a smaller band (~20 kDa). Use nuclear extracts for optimal detection and verify with positive controls.
• ELISA considerations: Optimize antibody coating concentration and use recombinant C/EBPB or peptide standards for quantitative assays. Pre-test cross-reactivity with other C/EBP family members if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials for C/EBPB research, including validated monoclonal antibodies, bulk reagents, and customized sourcing for rare targets. With a focus on supporting diagnostic manufacturers and laboratories, CamelBio bridges the gap between concept and clinical application through reliable supply and technical expertise.