Anti-CEBPA Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P53566

Core Product Specifications and Parameters

Biological Background: CEBPA Function and Localization

• CEBPA encodes a basic leucine zipper transcription factor that coordinates proliferation arrest and terminal differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and placenta.
  Related references: PMID:15107404, PMID:15589173, PMID:36228616
• The factor binds to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' and acts as a transcriptional activator on distinct target genes.
• It is essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP) and is critical for proper development of the liver and lung.
  Related references: PMID:24367003, PMID:8798745
• CEBPA is necessary for terminal adipocyte differentiation, required for postnatal maintenance of systemic energy homeostasis and lipid storage, and achieves this in part through E2F1 repression and interaction with the SWI/SNF complex.
  Related references: PMID:1935900, PMID:8090719, PMID:11672531, PMID:14660596
• In the liver, CEBPA regulates gluconeogenesis via functional cooperation with FOXO1 and modulates lipogenesis by transcriptionally synergizing with SREBF1.
  Related references: PMID:17627282, PMID:17290224
• The gene produces multiple isoforms: isoform 2 is the main active transcription factor, while isoform 3 can act as a dominant-negative with reduced transactivation activity and does not inhibit cell proliferation.
• CEBPA directly enhances ribosomal DNA transcription by interacting with RNA polymerase I-specific cofactors and inducing histone acetylation, and localizes to the nucleus and nucleolus.
• Post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation regulate CEBPA activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-117 of the mouse CEBPA N-terminal transactivation domain, a region conserved across human, mouse, and rat, which supports the reported cross-reactivity.
• When performing Western blotting, consider that alternative translation initiation and post-translational modifications may yield multiple bands; typical molecular weights for isoforms include 37 kDa (isoform 2), 36 kDa (isoform 3), and a potential phosphorylated form at 41 kDa.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), liver and adipose tissue serve as appropriate positive controls due to high CEBPA expression.
• This polyclonal antibody is suitable for ELISA applications; optimal working dilutions should be determined experimentally in the assay system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing IVD raw materials, offering a comprehensive portfolio of high-quality antibodies and reagents for diagnostic development. This CEBPA rabbit polyclonal antibody supports research in hematology, oncology, and metabolic diseases, enabling robust detection in multiple applications. From validated antibody pairs to optimized monoclonals and bulk ancillary supplies, CamelBio streamlines your path from concept to clinic.