Anti-C/EBPα Polyclonal Antibody for WB, ELISA - P49715

Core Product Specifications and Parameters

Biological Background: C/EBPα Function and Localization

• Protein identity and gene family: C/EBPα, encoded by CEBPA, is a basic leucine zipper transcription factor of the CCAAT/enhancer-binding protein family.
• DNA binding and transcription activation: It specifically binds the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' to activate transcription of target genes involved in differentiation and metabolism. Related references: PMID:11242107
• Cell differentiation and proliferation arrest: C/EBPα is essential for the terminal differentiation of myeloid progenitors, adipocytes, hepatocytes, and other cell types, enforcing proliferation arrest via E2F1 repression and SWI/SNF complex engagement. Related references: PMID:14660596
• Isoform function: An alternative isoform can act as a dominant-negative, binding DNA with reduced transactivation capacity and without inhibiting proliferation. Related references: PMID:14660596
• Tissue-specific roles: In the liver, it regulates gluconeogenesis and lipogenesis through FOXO1 and SREBF1 synergy; in adipose tissue, it coactivates FOXO1 to control adipokine expression.
• rDNA transcription and chromatin: C/EBPα enhances ribosomal DNA transcription by interacting with RNA polymerase I cofactors and inducing histone acetylation, linking it to nucleolar function and chromatin modification.
• Subcellular localization: It predominantly resides in the nucleus and nucleolus, consistent with its dual role in mRNA and rRNA synthesis.
• Post-translational regulation: Activity is modulated by phosphorylation, acetylation, and ubiquitination, as annotated in UniProt (keywords: Phosphoprotein, Acetylation, Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen consists of a synthetic peptide from the central region (aa 150–250) of human C/EBPα; sequence alignment suggests potential cross-reactivity with mouse C/EBPα, consistent with the listed cross-reactivity.
• For Western blotting, a band at approximately 38 kDa is expected; we recommend optimizing primary antibody dilution and including appropriate positive controls (e.g., HepG2 or 3T3-L1 lysates).
• For ELISA applications, this polyclonal antibody may be used as a detection reagent; consider pairing with a monoclonal capture antibody for sandwich ELISA development.
• Since C/EBPα is a transcription factor, nuclear extracts or whole-cell lysates may improve detection sensitivity.

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