Anti-CEBP Delta/CEBPD Rabbit Polyclonal Antibody for WB, ELISA - P49716

Core Product Specifications and Parameters

Biological Background: CEBP Delta Function and Localization

• CEBPD functions as a transcription activator that binds CCAAT and enhancer core motifs, crucially regulating genes involved in immune and inflammatory responses.
  Related references: PMID:16397300, PMID:1741402
• It enhances IL6 transcription both alone and as a heterodimer with CEBPB, contributing to cytokine production during inflammation.
  Related references: PMID:1741402
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications such as acetylation and ubiquitin-like conjugation (isopeptide bond) have been identified, which may modulate its activity.
• CEBPD is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors, characterized by a basic leucine zipper (bZIP) domain.
• UniProt annotates CEBPD with keywords including Acetylation, Activator, DNA-binding, Isopeptide bond, Nucleus, Proteomics identification, Reference proteome, Transcription, Transcription regulation, and Ubl conjugation, highlighting its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from the region spanning amino acids 50–150 of human CEBPD. Ensure that your assay conditions are optimized for detection of the native protein.
• For Western blot analysis, a predominant band at approximately 28 kDa is expected in human and mouse lysates, with possible additional bands due to post-translational modifications.
• ELISA performance may be influenced by coating antigen and antibody dilution; titrate the antibody to identify the optimal working concentration.
• When using mouse samples, verify cross-reactivity through a preliminary experiment, as the antibody is reported to recognize mouse CEBPD.
• Always include appropriate positive and negative controls to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with reliable access to IVD raw materials, including validated polyclonal and monoclonal antibodies against transcription factors like CEBPD. As a one-stop partner, we support your procurement of bulk antibody supplies, ancillary reagents, and rare-target sourcing—streamlining your workflow from early-stage research to diagnostic development. Whether you need consistent lot-to-lot performance or specialized reagents for immune and inflammatory disease studies, our team ensures efficient, quality-assured supply.