Anti-CEBP Delta/CEBPD Polyclonal Antibody for WB, ELISA - P49716

Core Product Specifications and Parameters

Biological Background: CEBP Delta/CEBPD Function and Localization

• CEBP Delta/CEBPD (CCAAT/enhancer-binding protein delta), also known as NF-IL6-beta, is a transcription factor encoded by the CEBPD gene that belongs to the C/EBP family of basic leucine zipper (bZIP) proteins.
• It functions as a transcriptional activator by binding to two distinct DNA motifs: the CCAAT homology in various promoters and the enhancer core homology in numerous enhancers, playing a key role in regulating gene expression (PubMed:16397300). Related references: PMID:16397300
• CEBPD is critically involved in the transcriptional control of immune and inflammatory response genes, including IL6, both as a homodimer and as a heterodimer with CEBPB (PubMed:16397300, PubMed:1741402). Related references: PMID:16397300 PMID:1741402
• The protein localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Post-translational modifications include acetylation and ubiquitination (isopeptide bond), as indicated by UniProt keywords such as "Acetylation", "Ubl conjugation", and "Isopeptide bond", which may regulate its activity and stability.
• Additional keywords "Proteomics identification" and "Reference proteome" denote its comprehensive annotation in human protein databases.

Experimental Guidance and Technical Tips

• The antibody was generated using a synthetic peptide corresponding to amino acids 50-150 of human CEBPD (NP_005186.2); this region is highly conserved, which may contribute to cross-reactivity with mouse and rat orthologs.
• For Western blot (WB) analysis, consider using whole cell or nuclear extracts, as CEBPD is a nuclear protein. Optimize blocking and detection conditions to visualize the expected 28 kDa band.
• In ELISA applications, validate the antibody against the target protein in the relevant sample system, and include appropriate positive and negative controls to ensure specificity.
• Since CEBPD functions as a transcription factor involved in immune responses, consider testing in cell lines or tissues stimulated with inflammatory cytokines (e.g., IL-6, LPS) to assess expression changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored for immunoassay development. For transcriptional regulation and immune response studies targeting CEBPD, we offer reliable sourcing solutions, including rare-target raw materials and technical consulting, to accelerate your diagnostic assay pipeline.