Anti-CEBP Delta/CEBPD Monoclonal Antibody for WB, ELISA - P49716

Core Product Specifications and Parameters

Biological Background: CEBP Delta/CEBPD Function and Localization

• CEBPD is a transcription activator that recognizes CCAAT and enhancer core DNA motifs, playing a key role in regulating genes involved in immune and inflammatory responses. Related references: PMID:16397300, PMID:1741402
• It enhances IL6 transcription both alone and as a heterodimer with CEBPB, contributing to cytokine signaling pathways. Related references: PMID:1741402
• The protein localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcription factor.
• Post-translational modifications, including acetylation and ubiquitination (isopeptide bond formation), have been identified, suggesting regulation of protein stability and activity.
• The predicted molecular weight is approximately 28 kDa (UniProt: 28,467 Da), and it has been detected in proteomics identification studies.
• Belongs to the C/EBP family of transcription factors, which are critical for cellular differentiation, proliferation, and stress responses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 50–150; consider this when designing blocking peptides or validating antibody specificity.
• For Western blot, the predicted molecular weight is 28 kDa; use appropriate positive controls (e.g., immune-stimulated cell lysates) and validate band size.
• For ELISA, optimize coating antigen concentration and antibody dilution by checkerboard titration; include relevant negative controls.
• Cross-reactivity has been confirmed with human and mouse; validate performance in your specific sample system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CEBPD antibody supports research into immune and inflammatory response pathways, and CamelBio can efficiently source rare-target raw materials for specialized diagnostic and research applications.