Anti-CEACAM6 Polyclonal Antibody for WB, ELISA - P40199

Core Product Specifications and Parameters

Biological Background: CEACAM6 Function and Localization

• CEACAM6 (Carcinoembryonic antigen-related cell adhesion molecule 6) is a member of the CEA family, also known as NCA, CD66c, and CEAL. It is a GPI-anchored glycoprotein expressed on the cell surface.
• Functions as a cell adhesion molecule, mediating both homophilic and heterophilic intercellular adhesion in a calcium- and fibronectin-independent manner (PubMed:11590190, PubMed:16204051, PubMed:2022629). Heterophilic interaction with CEACAM8 occurs specifically in activated neutrophils (PubMed:8776764). Related references: PMID:11590190 PMID:16204051 PMID:2022629 PMID:8776764
• Plays a significant role in tumor progression and cell migration, particularly adhesion to endothelial cells (PubMed:16204051, PubMed:10910050). Related references: PMID:16204051 PMID:10910050
• Involved in neutrophil adhesion to cytokine-activated endothelial cells, contributing to inflammatory responses (PubMed:1378450). Related references: PMID:1378450
• Localized at the apical cell membrane and cell surface, consistent with its role in cell-cell interactions and signaling.
• Expressed in neutrophils, columnar epithelial and goblet cells of the colon, and numerous tumor cell lines (at protein level) (PubMed:1378450, PubMed:10436421, PubMed:16204051). Related references: PMID:1378450 PMID:10436421 PMID:16204051
• The protein contains immunoglobulin domains, disulfide bonds, and a GPI anchor; it is classified under keywords including cell adhesion, oncogene, glycoprotein, and membrane protein. Its predicted molecular weight is approximately 37 kDa, though actual migration may vary due to glycosylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 35–320 of human CEACAM6, covering the extracellular domain. This region is ideal for detecting both full‑length and shed forms of the protein.
• For Western blotting, consider using reducing and/or non‑reducing conditions, as the protein may form disulfide‑linked dimers or multimers. The expected monomeric band is around 37 kDa; however, extensive glycosylation can shift the apparent molecular weight to ~50–60 kDa in many cell types.
• In ELISA, the antibody may be suitable for direct or capture formats; validate pairing with a detection antibody and confirm specificity using recombinant CEACAM6 or CEACAM family cross‑reactivity controls.
• When analyzing mouse samples, verify cross‑reactivity through a pilot experiment, as the antibody is reported to recognize mouse CEACAM6 but may exhibit different affinity.
• Always include appropriate positive controls, such as human neutrophil lysates or colon carcinoma cell lines (e.g., HT‑29, LS‑174T), and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, from concept to clinic. This CEACAM6 antibody supports cell adhesion, immunology, and oncology research—fields where we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Connect with us to streamline your diagnostic development with reliable, high‑quality reagents tailored to your workflow.