Anti-CEACAM6 Monoclonal Antibody for WB, IF-P, ELISA - P40199

Core Product Specifications and Parameters

Biological Background: CEACAM6 Function and Localization

• CEACAM6 (CD66c, NCA) is a cell surface glycoprotein belonging to the carcinoembryonic antigen (CEA) family, encoded by the CEACAM6 gene.
• It mediates calcium- and fibronectin-independent homophilic cell adhesion, as well as heterophilic interactions with CEACAM5 and CEACAM8, contributing to intercellular contacts. Related references: PMID:2022629 PMID:16204051 PMID:11590190
• Plays a role in neutrophil adhesion to cytokine-activated endothelial cells, facilitating immune cell trafficking. Related references: PMID:1378450
• Involved in tumor progression, promoting cell migration and adhesion to endothelial cells, which may support metastatic spread. Related references: PMID:16204051 PMID:10910050 PMID:11590190 PMID:1378450
• Localized primarily to the cell membrane and apical cell surface, with a glycosylphosphatidylinositol (GPI) anchor.
• Contains immunoglobulin-like domains and forms disulfide bonds; subject to N-glycosylation.
• Expressed in neutrophils, colon epithelial and goblet cells, and various tumor cell lines. Related references: PMID:1378450 PMID:10436421 PMID:16204051
• Keywords also highlight roles in apoptosis and oncogenesis, with a known 3D structure.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 200–300 of human CEACAM6, likely targeting an extracellular domain. For Western blotting (WB), consider using reducing conditions and whole-cell lysates from CEACAM6-expressing cell lines (e.g., human colon carcinoma HT-29 or MCF-7). The predicted molecular weight is ~37 kDa, though glycosylation may cause band shifts.
• For immunofluorescence on paraffin-embedded sections (IF-P), validate in appropriate tissue types and optimize antigen retrieval protocols. The antibody may also be suitable for ELISA applications; however, cross-reactivity with related CEACAM family members (e.g., CEACAM5, CEACAM8) should be carefully evaluated in assay development.
• Given the reported cross-reactivity with mouse and rat, this antibody may be useful for studies in these species, but additional validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. For CEACAM6-related projects—spanning cell adhesion, tumor marker research, and immunoassay development—we offer validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. Whether you need bulk supply or assistance with rare target sourcing, our one-stop platform supports your journey from concept to clinic.