Anti-CEACAM6 Monoclonal Antibody for WB, ELISA - P40199

Core Product Specifications and Parameters

Biological Background: CEACAM6 Function and Localization

• CEACAM6 (CD66c), also designated NCA or CEAL, is a cell surface glycoprotein belonging to the carcinoembryonic antigen (CEA) family.
• It mediates calcium- and fibronectin-independent intercellular adhesion, engaging in homophilic and heterophilic interactions with CEACAM5 and CEACAM8 (PubMed:11590190, PubMed:16204051, PubMed:2022629). Related references: PMID:11590190, PMID:16204051, PMID:2022629.
• CEACAM6 promotes neutrophil adhesion to cytokine-activated endothelial cells and binds CEACAM8 on activated neutrophils, contributing to inflammatory processes (PubMed:1378450, PubMed:8776764). Related references: PMID:1378450, PMID:8776764.
• It plays a role in tumor progression by enhancing cell migration and adhesion to endothelial cells, and its expression is elevated in numerous tumor cell lines (PubMed:16204051). Related references: PMID:16204051.
• The protein is anchored to the apical cell membrane via a GPI linkage and features immunoglobulin domains, disulfide bonds, and glycosylation sites.
• CEACAM6 exhibits tissue-specific expression in neutrophils, colon epithelial cells, and goblet cells (PubMed:1378450, PubMed:10436421). Related references: PMID:1378450, PMID:10436421.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 35–320), suggesting the antibody recognizes native and denatured CEACAM6; consider initial use in denaturing Western blots with a ~37 kDa expected band.
• For ELISA, coating with recombinant CEACAM6 protein or cell‑based assays may be suitable; optimize coating concentrations and detection conditions for your specific system.
• This antibody is raised against human CEACAM6; cross-reactivity with rodent orthologs is not expected, but verify if needed with species-appropriate samples.
• Given the GPI-anchored nature of the target, include appropriate lysis buffers or enzymatic release strategies if studying membrane localization.

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