Anti-CEACAM5/CD66e Rabbit Monoclonal Antibody () for Flow Cytometry - P06731

Core Product Specifications and Parameters

Biological Background: CEACAM5 Function and Localization

• CEACAM5, also known as carcinoembryonic antigen (CEA) or CD66e, is a GPI-anchored glycoprotein belonging to the immunoglobulin superfamily.
• It mediates homophilic and heterophilic cell adhesion with other CEACAM family members, such as CEACAM6, contributing to tissue organization and signaling. Related references: PMID:2803308
• CEACAM5 acts as an oncogene, promoting tumor progression and inducing resistance to anoikis in colorectal carcinoma cells. Related references: PMID:10910050 PMID:10864933
• It serves as a receptor for E. coli Dr adhesins, with binding leading to homodimer dissociation.
• The protein localizes to the apical cell membrane and cell surface.
• CEACAM5 is expressed in columnar epithelial and goblet cells of the colon, and is found in adenocarcinomas of the digestive system and fetal colon. Related references: PMID:10436421
• It features immunoglobulin domains, disulfide bonds, and undergoes alternative splicing, with a molecular weight of approximately 76.8 kDa.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , a red fluorescent dye with excitation/emission maxima ~590/617 nm, suitable for flow cytometry. Protect from light and store at 2–8°C.
• The immunogen includes the extracellular region (aa 35–685) of human CEACAM5, encompassing multiple Ig-like domains; consider potential cross-reactivity with closely related CEACAMs in your assay design.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and include appropriate isotype and fluorescence-minus-one (FMO) controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to validated IVD raw materials, including monoclonal antibodies like this anti-CEACAM5/CD66e product. As a B2B sourcing partner, we supply optimized antibody pairs, bulk ancillary reagents, and assist with rare-target raw material procurement to support your cancer biomarker assay development—from concept through clinical application.