Anti-CEACAM5 Polyclonal Antibody for WB, ELISA - P06731

Core Product Specifications and Parameters

Biological Background: CEACAM5 Function and Localization

• CEACAM5 (Carcinoembryonic antigen-related cell adhesion molecule 5), also known as CEA or CD66e, is a cell surface glycoprotein that plays roles in cell adhesion, intracellular signaling, and tumor progression. Related references: PMID:10864933, PMID:10910050, PMID:2803308
• It mediates homophilic and heterophilic cell adhesion with other CEACAM family members, such as CEACAM6.
• CEACAM5 functions as an oncogene, promoting tumor progression and inducing resistance to anoikis in colorectal carcinoma cells. Related references: PMID:10910050
• It acts as a receptor for E. coli Dr adhesins, leading to dissociation of the homodimer.
• Localized primarily to the apical cell membrane and cell surface via a GPI anchor.
• Highly expressed in columnar epithelial and goblet cells of the colon, with elevated levels in adenocarcinomas of the digestive system and fetal colon. Related references: PMID:10436421
• The protein features immunoglobulin-like domains, multiple disulfide bonds, and extensive glycosylation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-702 of human CEACAM5, encompassing the entire extracellular domain; consider this full-length coverage for applications where native conformation recognition is critical.
• For Western blot, consider testing whole-cell lysates from human colorectal carcinoma cell lines (e.g., HT-29, LS174T) where CEACAM5 expression is well documented; validate band identity with appropriate controls.
• ELISA may be suitable for detecting soluble CEA in serum or cell culture supernatants; ensure optimization of coating and detection conditions.
• Cross-reactivity with mouse has been reported; verify in relevant mouse tissues or cell lines, and consider sequence alignment to assess epitope conservation.
• Given its GPI-anchored nature, include gentle lysis conditions to preserve membrane localization if studying surface expression.

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CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including high-quality antibodies targeting key oncology biomarkers like CEACAM5. From validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents, our portfolio supports every stage of assay development. Contact us for sourcing rare targets and customized solutions tailored to your clinical development needs.