Anti-CEACAM5 Monoclonal Antibody for WB, IHC-P, ELISA - P06731

Core Product Specifications and Parameters

Biological Background: CEACAM5 Function and Localization

• Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression.
  Related references: PMID:10864933 PMID:10910050 PMID:2803308
• Mediates homophilic and heterophilic cell adhesion with CEACAM6.
  Related references: PMID:2803308
• Acts as an oncogene by promoting tumor progression and inducing resistance to anoikis of colorectal carcinoma cells.
  Related references: PMID:10910050
• Functions as a receptor for E.coli Dr adhesins, which leads to dissociation of the homodimer.
• Localizes to the cell membrane, apical cell membrane, and cell surface, consistent with its role in cell adhesion and signaling.
• Expressed in columnar epithelial and goblet cells of the colon; found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  Related references: PMID:10436421
• Features Ig-like domains, a GPI-anchor, disulfide bonds, and undergoes alternative splicing; classified as an oncogene and reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 35-685, covering the majority of the extracellular domain; this design may provide broad epitope coverage.
• For Western blotting, the predicted molecular weight is approximately 77kDa, but extensive glycosylation may cause the band to appear at a higher molecular weight; validate in the relevant sample system.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), consider optimizing antigen retrieval conditions and validate in known positive tissues such as human colon adenocarcinoma.
• For ELISA applications, test both direct and sandwich formats; pairing with another CEACAM5-specific antibody may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized one-stop supplier for IVD raw materials, CamelBio supports diagnostic manufacturers and researchers with high-quality validated antibodies like this anti-CEACAM5 clone, along with complementary antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Whether you are developing assays for cancer biomarkers or exploring cell adhesion mechanisms, CamelBio can source tailored raw materials for your entire workflow.