Anti-CEACAM5 (CEA/CD66e) Rabbit Polyclonal Antibody for WB, ELISA - P06731

Core Product Specifications and Parameters

Biological Background: CEACAM5 Function and Localization

• CEACAM5 (carcinoembryonic antigen, CEA, CD66e) is a cell surface glycoprotein involved in cell adhesion, intracellular signaling, and tumor progression. Related references: PMID:10864933, PMID:10910050, PMID:2803308
• It acts as an oncogene by inducing resistance to anoikis in colorectal carcinoma cells, thereby promoting tumor progression. Related references: PMID:10910050
• Mediates homophilic and heterophilic cell adhesion with other CEACAM family members, such as CEACAM6, contributing to tissue architecture and cell-cell communication.
• At the protein level, CEACAM5 is expressed in columnar epithelial and goblet cells of the colon and is highly abundant in adenocarcinomas of the digestive tract and fetal colon. Related references: PMID:10436421
• Functions as a receptor for E.coli Dr adhesins; bacterial binding triggers dissociation of the CEACAM5 homodimer.
• Subcellular localization: Predominantly found on the cell membrane, enriched at the apical cell membrane in polarized epithelial cells, and present on the cell surface.
• Structurally, CEACAM5 features multiple immunoglobulin-like domains, a GPI-anchor for membrane attachment, extensive glycosylation, and intrachain disulfide bonds, which support its adhesive and signaling roles.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 35-685) covers most of the extracellular region, likely enabling recognition of both native and denatured CEACAM5; this design is well-suited for WB and ELISA.
• For WB: Use denaturing conditions. The mature glycoprotein migrates at ~77 kDa, but glycosylation may cause band shifts. Validate with a positive control such as colon carcinoma cell lines (e.g., HT-29, Caco-2).
• For ELISA: This polyclonal antibody may be employed as a detection antibody after appropriate titration, using recombinant CEACAM5 protein as a standard and a matched capture antibody.
• Cross-reactivity has been demonstrated for human; assess other species if relevant to your experimental system.
• As a polyclonal antibody, verify lot-to-lot consistency when used in quantitative or regulated assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, labs, and research institutes with a comprehensive portfolio of IVD raw materials and technical services, spanning concept to clinic. For CEACAM5-related projects, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are developing a colon cancer biomarker assay or exploring CEACAM5’s role in tumor progression, CamelBio streamlines procurement and provides expert guidance to accelerate your development.